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N1-Methylpseudouridine 5'-Triphosphate is a modified nucleoside triphosphate used in RNA research and synthesis. It contains a methyl group on the N1 position of the pseudouridine nucleobase. This analog is cell permeable and can be incorporated into RNA by polymerases. It is supplied as a lithium salt at ≥95% purity by TriLink BioTechnologies. The product has a molecular weight of 491.15 g/mol and is soluble up to 100 mM in water or buffer. It can be used to study RNA structure and function.
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CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. They have been shown to induce a coordinated set of immune responses based on the activation of immune cells primarily involved in the recognition of these molecules.
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